Poolesville is a town located in the Maryland region of the United States. During the most recent census, Poolesville had a population of 4,883. Poolesville is the 167th most poplulated city in the Maryland region, the 6,359th most populated city in the United States and the 30,377th most populated city in the world. Poolesville is in the America/New York timezone which is 4 hours behind UTC. Poolesville adjusts the hour with daylight savings time during the winter months.